Do fecal bile acid analyses allow taxonomic discrimination between Neotropical mustelids?

Lana R. Almeida; Ana Maria O. Mastella; Marina A. Alves; Rafael Garret; Maria João R. Pereira

doi:10.31687/SaremNMS23.6.4

Autores/as

Lana R. Almeida Bird and Mammal Evolution, Systematics and Ecology Lab, Programa de Pós-Graduação em Biologia Animal, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil. Laboratório de Estudos Planctônicos e Divulgação Científica, Programa de Pós-Graduação em Ciências e Tecnologias Ambientais, Universidade Federal do Sul da Bahia / Instituto Federal da Bahia, Porto Seguro, BA, Brazil.
Ana Maria O. Mastella Bird and Mammal Evolution, Systematics and Ecology Lab, Programa de Pós-Graduação em Biologia Animal, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil. Programa de Pós-Graduação em Biologia Animal, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
Marina A. Alves Universidade Federal do Rio de Janeiro, Metabolomics Laboratory (LabMeta – LADETEC/IQ – UFRJ), Chemistry Institute, Rio de Janeiro, RJ, Brazil. Universidade Federal do Rio de Janeiro, Walter Mors Institute of Research on Natural Products, Rio de Janeiro, RJ, Brazil.
Rafael Garret Universidade Federal do Rio de Janeiro, Metabolomics Laboratory (LabMeta – LADETEC/IQ – UFRJ), Chemistry Institute, Rio de Janeiro, RJ, Brazil.
Maria João R. Pereira Bird and Mammal Evolution, Systematics and Ecology Lab, Programa de Pós-Graduação em Biologia Animal, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil. Programa de Pós-Graduação em Biologia Animal, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.

DOI:

https://doi.org/10.31687/SaremNMS23.6.4

Palabras clave:

chemical profiles, Mustelidae, Neotropics, taxonomy

Resumen

The use of fecal samples analysis to differentiate species is becoming more frequent in recent years. We performed a semi-quantitative fecal bile acid analysis of five Neotropical mustelids to access the diversity of bile acids, evaluating their taxonomic discriminatory potential. Fecal samples were collected from captive specimens and analyzed through liquid chromatography high-resolution mass spectrometry (LC-HRMS). We found significant differences between the analyzed species in four bile acids, supporting our method as a promising tool for taxonomic discrimination among mammals. Bile acids are endogenous compounds fundamental in biochemical pathways, so detecting interspecific differences may be relevant to understanding the physiology, health status and metabolic responses of mustelids to environmental changes.

Citas

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